fluorescent staining method meaning in Chinese

荧光染色法

Materials and methods the mouse , golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation , respectively , and ar was induced by progesterone after capacitation , then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method . the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf , then the fertilization rate was observed . the 1 - cell , 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin , then the rate of blastocysts was recorded
方法取小鼠精子10份、金黄地鼠精子6份、人新鲜精液标本10份及人冷冻精液标本9份，分别与不同浓度内毒素共孵育进行体外获能和孕酮诱导的顶体反应，应用金霉素和dna结合的荧光染料hoechest33258双重荧光染色法检测精子的获能率和顶体反应率；小鼠体外受精实验的精卵结合环节培养液中加入不同浓度的内毒素，观察受精情况并记录受精率；取小鼠1 -细胞胚胎、 2 -细胞胚胎和去卵透明带2 -细胞胚胎，与不同浓度内毒素共孵育进行体外培养，观察体外发育情况并记录囊胚率。